OpinionPolitics

Chinese Conducted Research on Monkeypox at Wuhan

A paper showing that the Chinese conducted research with the Monkeypox virus back in February of 2022 has been sent to us. Two of the Professors mentioned in the paper that shared their expertise with the researchers have worked at the Wuhan Institute of Virology. All of the researchers are from the WIV.

“This work was supported by the National Science and Technology Major Project of China (2018ZX10711001-006) and the Key Research Program of the Chinese Academy of Sciences (KJZD-SW-L06-02). We acknowledge Prof. Manli Wang and Dr. Hengrui Hu for the generous sharing of their expertise in the TAR assembly.”

Monkeypox research paper

The Chinese conducted research

TAR stands for “transformation-associated recombination.” It is a cloning process that uses yeast to recover a DNA strand from a complex genome…like monkeypox, for example. It is the only method available at this time to obtain a large strand of DNA. Because China has never had an outbreak of Monkeypox, the researchers had to find something to use.

Monkey poxviruses (MPXVs) are large DNA viruses with reported genome sizes ranging from 190,083 to 206,372 bp in length. As a member of the Orthopoxvirus genus in the family Poxviridae, MPXV is subdivided into the West African and Congo Basin clades. The latter is more pathogenic and has been reported to infect humans in various parts of the world [reviewed in (Brown and Leggat, 2016)]. Quantitative polymerase chain reaction (qPCR) is the gold standard for the detection of orthopoxvirus (including MPXV). For pan-orthopoxviruses detection, the E9L (DNA polymerase) gene has been shown to be an excellent target for qPCR assays (Kulesh et al., 2004). For MPXV detection, Li et al. reported that the C3L (complement-binding protein) gene could be used as the qPCR target for the MPXV Congo Basin strain (Li et al., 2010). Since MPXV infection has never been associated with an outbreak in China, the viral genomic material required for qPCR detection is unavailable. In this report, we employed dual-selective TAR to assemble a 55-kb MPXV genomic fragment that encompasses E9L and C3L, two valuable qPCR targets for detecting MPXV or other orthopoxviruses. The dual-selective TAR assembly showed a zero rate of vector recircularization and an average legitimate assembly yield of 79%, demonstrating that PADH1-URA3, serving as a negative selective marker, can optimize TAR assembly by abolishing vector recircularization.

Monkeypox Research Paper

The experiment cloned, or synthesized 4 Monkeypox DNA strands into one large fragment. Why? The researchers were hoping to create a template to detect Monkeypox. According to the study, the researchers deliberately only used a portion of the DNA strand to keep it from developing into an infectious pathogen.

“The primary purpose of assembling a fragment of the MPXV genome is to provide a nucleotide template for MPXV detection….In this study, although a full-length viral genome would be the ideal reference template for detecting MPXV by qPCR, we only sought to assemble a 55-kb viral fragment, less than one-third of the MPXV genome. This assembly product is fail-safe by virtually eliminating any risk of recovering into an infectious virus while providing multiple qPCR targets for detecting MPXV or other Orthopoxviruses (Li et al., 2010).”

Monkeypox research paper

On May 20, the WHO called an emergency meeting over the Monkeypox outbreak due to it being “atypical.” The cases presented so far were not related to the countries that normally have the disease, as we have previously reported. The researchers named in the study seemed to be genuinely trying to avoid a disaster with their research.

There are numerous theories floating around out there at this time. Because this Chinese conducted research paper was sent to us, we reported on it. There is no information at this time that accuses these scientists of “leaking” any of their synthesized monkeypox from the lab. It is interesting that the Wuhan Institute of Virology conducted the research.

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H/T Uncle Sam’s Misguided Children

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Faye Higbee

Faye Higbee is the columnist manager for Uncle Sam's Misguided Children. All of her posts on Conservative Firing Line are also posted on Uncle Sam's.

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